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Most relevant scientific articles
• CarrasCosa l.g., sina a.a.i., PalanisaMy r., sePúlVeDa B., otte M.a., rauF s. et al . Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methyla- tion . Chemical Communications . 2014;50(27):3585-3588 .
• estéVez M.-C., otte M.a., sePúlVeDa B., leChuga l.M. Trends and challenges of refractometric nanoplas- monic biosensors: A review . Analytica Chimica Acta . 2014;806:55-73 .
• Mauriz e., CarBaJo-PesCaDor s., orDónez r., garCía-FernÁnDez M.C., Mauriz J.l., leChuga l.M. et al . On-line surface plasmon resonance biosensing of vascular endothelial growth factor signaling in intact-human hepatoma cell lines . Analyst . 2014;139(6):1426-1435 .
• olguin y., CarrasCosa l.g., leChuga l.M., young M. The effects of lipids and surfactants on TLR5-pro- teoliposome functionality for flagellin detection using surface plasmon resonance biosensing . Talanta . 2014;126:136-144 .
• soler M., estéVez M.C., alVarez M., otte M.a., sePúlVeDa B., leChuga l.M. Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies .Sensors (Basel, Swit- zerland) . 2014;14(2):2239-2258 .
Highlights
Institution: Agencia Estatal Consejo Superior De Investigaciones Científicas Contact: Institut Català de Nanociència i Nanotecnologia . Campus de la UAB, Edificio ICN2 . Bellaterra-Cerdanyola del Vallés . 08193 Barcelona · E-mail: laura .lechuga@cin2 .es Web: nanob2a .cin2 .es
In the research line of integrated silicon nanophotonic biosensors, important milestones have been reached for the implementation of a sensitive, affordable, hand-held and portable point-of-care device . The ultrasensitive limit of detection of this technology at the pM-fM level is far beyond the state-of-the- art and we have demonstrated the detection of a few number of infectious microorganisms directly in patient´s samples . A technological transfer plan with a private company has been developing during 2014 . Several new projects have been granted in 2014 related to this research line:
• COLONTEST: Diseño y puesta a punto de kits para el diagnóstico del cáncer de colon en sangre basa- dos en plataformas multiplex . (RTC-2014-1518-1)
• RAIS: Scalable, point-of-care and label free microarray platform for rapid detection of Sepsis (UE. H2020-ICT-2014-1-644956)
The utility of our nanophotonic biosensing techniques for real bioanalytical applications has been suc- cessfully demonstrated . We are focusing in the point-of-care detection of diseases as for example Ma- laria (collaboration with FIND diagnostics Foundation) or Tuberculosis (POCKET EU project), early detec- tion of several types of cancer as colorectal cancer (COLONTEST national project); early detection of liver complications (CIBER internal project); monitoring of celiac or allergic patients directly in their human fluids, among others (most of them in collaboration with private companies) . In the environmental field, we are focusing in the early detection of toxic pollutants as pesticides, antibiotics or alga toxins (BRAA- VOO EU project) .
We have successfully developed in 2014 the fundamental research line in Molecular Biology using our nanobiosesing technology for the deciphering of alterations in the cellular pathways, including alterna- tive splicing of RNA, and epigenetics modifications (DNA metylation or microRNA release) .
2014 indicators to be noted: 7 international publications, 1 industrial contract, 21 invited contributions to conferences and courses .
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