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• t m, G i, m B, e r, F C. DNA origami as a DNA re-
Most relevant intoréálleGoAnninGritJAàBreGA
pair nanosensor at the single-molecule level.Angew Chem Int Ed Engl. 2013 Jul
scientific 22;52(30):7747-50.

articles
• terrAzAs m, AlAGiA A, FAustino i, orozCo m, eritJA r. Functionalization of the 3’-ends of
DNA and RNA strands with N-ethyl-N-coupled nucleosides: a promising approach to

avoid 3’-exonuclease-catalyzed hydrolysis of therapeutic oligonucleotides.Chembio-
chem. 2013 Mar 4;14(4):510-20.

• u-u B, G s, B Jv, m C, e r, G Fm .. Double-tailed li-
GArteriBeriJAlvoustoArtínritJAoñiet Al
pid modification as a promising candidate for oligonucleotide delivery in mammalian
cells.Biochim Biophys Acta. 2013 Oct;1830(10):4872-84.

• G-P i, v-C e, l r, A A, e r, G C .. Carbo-
ómezintoenGutlimentuCAsviñóritJAonzálezet Al
hydrate-DNA interactions at G-quadruplexes: folding and stability changes by atta-
ching sugars at the 5’-end.Chemistry. 2013 Feb 4;19(6):1920-7.

• F r, A r, B A, G r, e r, F dm .. Structure and
erreirArtAlienoitArGAlloritJAerGusonet Al
stability of human telomeric G-quadruplex with preclinical 9-amino acridines.PLoS

One. 2013;8(3):e57701.

Highlights
Development a novel modification for protecting the 3’- ends of oligonucleoti-
des against degradation by nucleases. The modification, N-ethyl-N-coupled dinu-

cleosides, has been tested in DNA and RNA yielding nuclease-stable oligonucleo-
tides without disturbing the inhibitory properties of siRNA.

Identification of new compounds that inhibit human apurinic endonuclease (Ape1)

by using docking-based virtual screening techniques. These compounds have ac-

tivities in the low to the medium micromolar range and potentiate the cytotoxicity
of the chemotherapeutic agents paving the road for improving chemotherapy by

fighting drug resistant mechanisms.

In a recent work, DNA origami technology was used to visualize the effect of DNA

structure on the binding of aptamers to thrombin. A number of aptamers that
have a structure that provides quadruplex affinity to thrombin are arranged on

the right side of a flat DNA structure. On the left side the same sequences are
placed with a modification that prevents the formation of quadruplex. By atomic

force microscopy (AFM) thrombin binding to quadruplex structures is observed.

Chemical modification introduced in the left side of the origami can be repair by
an enzyme involved in the resistance of cancer cells to chemotherapy. This sys-

tem allows also visualizing the repair activity of this DNA repair enzyme involved

in cancer.

Novel formulations based on cationic lipids developed by our group have been
successfully used for gene therapy in eye diseases.

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